常用荧光染料的激发波长和发射波长:
|
荧光染料 Fluorescent Dye |
激发波长（nm） Excitation |
发射波长（nm） Emission |
|
Cy2 |
489 |
506 |
|
GFP(Red Shifted) |
488 |
507 |
|
YO-PRO-1 |
491 |
509 |
|
YOYO-1 |
491 |
509 |
|
Calcein |
494 |
517 |
|
FITC |
494 |
518 |
|
FluorX |
494 |
519 |
|
Alexa 488 |
490 |
520 |
|
Rhodamine 110 |
496 |
520 |
|
ABI,5-FAM |
494 |
522 |
|
Oregon Green 500 |
503 |
522 |
|
Oregon Green 488 |
496 |
524 |
|
Rlbo Green |
500 |
525 |
|
Rhodamine Green |
502 |
527 |
|
Rhodamine 123 |
507 |
529 |
|
Magnesium Green |
506 |
531 |
|
Calcium Green |
506 |
533 |
|
TO-PRO-1 |
514 |
533 |
|
TOTO-1 |
514 |
533 |
|
ABI,JOE |
520 |
548 |
|
BODIPY 530/550 |
530 |
550 |
|
Dil |
549 |
565 |
|
BODIPY R |
542 |
568 |
|
BODIPY 558/568 |
558 |
568 |
|
BODIPY 564/570 |
564 |
570 |
|
Cy3 |
550 |
570 |
|
Alexa 546 |
555 |
570 |
|
TRITC |
547 |
572 |
|
Magnesium Orange |
550 |
575 |
|
Phycoerythrin, R&B |
565 |
575 |
|
Rhodamine Phalloidin |
550 |
575 |
|
Calcium Orange |
549 |
576 |
|
Pyronin Y |
555 |
580 |
|
Rhodamine B |
555 |
580 |
|
ABI,TAMRA |
560 |
582 |
|
Rhodamine Red |
570 |
590 |
|
Cy3.5 |
581 |
596 |
|
ABI,ROX |
588 |
608 |
|
Calcium Crimson |
590 |
615 |
|
Alexa 594 |
590 |
615 |
|
Texas Red |
595 |
615 |
|
Nile Red |
549 |
628 |
|
YO-PRO-3 |
612 |
631 |
|
YOYO-3 |
612 |
631 |
|
R-Phycocyanin |
618 |
642 |
|
C-Phycocyanin |
620 |
648 |
|
TO-PRO-3 |
642 |
660 |
|
TOTO-3 |
642 |
660 |
|
DiD DilC’(5) |
644 |
665 |
|
Cy5 |
649 |
670 |
|
Thiadicarbocyanine |
651 |
671 |
|
Cy5.5 |
675 |
694 |