1. first, open the wheat omics(excellent web),use the JBrowse of genome，locate your region,and select the left predicted_ssr database ,and then download the sqn format.
2. then,the left position of the ssr minus 250bp,and the right plus 250bp,and the format for extract the sequence is chr1:100-600
3. .then,use the tools of wheat omics (get sequence),copy the format(chr1:100-600)
4. then,open the batchprimer3 website
5. then,select the ssr screening and primers
6. then,paste the sequence
7. then,click pick primers
wait...
8. save the excel file,and modify it
9. finally,send this to company