tophat -p 4 -G filter.gtf
-o /SRR222
/index
SRR222.fastq
tophat -p 4 -G filter.gtf
-o /SRR222
/index
SRR717.fastq,SRR718.fastq
tophat -p 4 -G filter.gtf
-o /SRR222
/index
SRR669_1.fastq
SRR669_2.fastq
Usage:
tophat [options] <bowtie_index> <reads1[,reads2,...]> [reads1[,reads2,...]]
[quals1,[quals2,...]] [quals1[,quals2,...]]
Usage:tophat [options]* <genome_index_base> <reads1_1[,...,readsN_1]> [reads1_2,...readsN_2]
查看tophat的帮助文件。